These SHP-2 actions are associated with augmented AKT signaling. In SHR Ang II-stimulated PTP oxidation/inactivation is enhanced, basal SHP-2 expression is reduced, and Ang II-induced PTP/SHP-2 phosphorylation is blunted. Hence, Ang II regulates PTP/SHP-2 in VSMCs through AT(1)R and Nox1-based NAD(P)H oxidase via two mechanisms, oxidation and phosphorylation. Nox1 downregulation attenuated Ang II-mediated AKT activation in SHRs. SHP-2 knockdown resulted in increased AKT phosphorylation, without effect on ERK1/2 or p38MAPK. Ang II stimulation increased activation of ERK1/2, p38MAPK, and AKT, with enhanced effects in SHR. Ang II-induced SHP-2 effects were inhibited by valsartan (AT(1)R blocker), apocynin (NAD(P)H oxidase inhibitor), and Nox1 siRNA. Ang II increased SHP-2 phosphorylation and activity with blunted responses in SHRs. Basal SHP-2 content was lower in SHRs versus WKY. Ang II induced oxidation of multiple PTPs, including SHP-2. SHP-2 and Nox1 were downregulated by siRNA. SHP-2 expression and activity were evaluated by immunoblotting and by a PTP activity assay, respectively. PTP oxidation was assessed by the in-gel PTP method. We propose that Ang II induces phosphorylation of growth signaling kinases by redox-sensitive regulation of protein tyrosine phosphatases (PTP) in VSMCs and that augmented Ang II signaling in spontaneously hypertensive rats (SHRs) involves oxidation/inactivation and blunted phosphorylation of the PTP, SHP-2. Angiotensin II (Ang II) signaling in vascular smooth muscle cells (VSMCs) involves reactive oxygen species (ROS) through unknown mechanisms. The image info data that follows is 40 bytes in. Text: Header: 40 bytes This block of bytes tells the application detailed information about the image, which will be used to display the image on the screen. Text: Stability -25 +25 ppm Operating Temperature - 40 +85 Â☌ +125 Â☌ 60 % Storage Temperature -55 Duty Cycle 50% Vcc 40 50 Rise Times TR From 10% to 90, CL 15.0 pF Tstup 4.0 mS Low Level Output Voltage Vol 0.4 V High Level HC49U KDS 8 MHZ crystal KDS Crystals KDS 16 MHZ crystal KDS 12 MHZ crystal KDS Crystals oscillator Hosonic 20 MHz crystal KDS Siward KDS Crystals 12 MHZ KDS 25 MHZ crystal D IS Note (2): The temperature of panel display surface, Operation 0 â for 240h (1) (2) High Temperature/Humidity Operation 40 â / 90%RH for 168h, mA (1),(2),(3) IPEAK - 40 100 mA (1),(2),(3) DC/DC Inrush Current Ta = 25Ībstract: KDS Crystals KDS 16 MHZ crystal KDS 12 MHZ crystal KDS Crystals oscillator Hosonic 20 MHz crystal KDS Siward KDS Crystals 12 MHZ KDS 25 MHZ crystal Text: Specification Pin numbers Golden Finger 40 Pitch (mm) PL Item AY Table 1-3 (1) S, Â✬ (1), (2) IN Note (1): C. (b) Wet-bulb temperature should be 39 Â✬ Max. (Ta ⦠40 Â✬), where Ta is ambient temperature. Text: FPC Specification Item Pitch (mm) Golden Finger Rev.: 04 Pin numbers 40 0.5 Page, Temperature TOP 0 +50 Â✬ (1), (2) Note (1): (a) 90 %RH Max. PL, Operation 0 â for 240h (1) (2) High Temperature/Humidity Operation 40 â / 90%RH for 168h, voltage VNEG +0.3 to -20 V VPOS-VNEG 40 V 40 V 0 to +50 ËC Max.
AY S (b) Wet-bulb temperature should be 39 Â✬ Max. Text: ) Golden Finger 40 0.5 Connector Note STARCONN 089H40 or Compatible PE R VA SI, TOP 0 +50 Â✬ (1), (2) C. PDI+40 Datasheets Context Search Catalog Datasheet
0 kommentar(er)
